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Clenbuterol is well-known to cause symptoms such as rapid heart rate (tachycardia), palpitations, tremors, anxiety, lowered blood potassium (hypokalemia), and elevated blood sugar (hyperglycemia). Adverse effects happen more often with the large doses used for performance enhancement and weight loss. SERS is a good detection scheme for the rapid detection and identifi cation of RAC and CL residues in pork. Keywords: ractopamine, clenbuterol hydrochloride, surface enhanced Raman spectroscopy, pork, rapid detection and identifi cation. Ractopamine (RAC) and clenbuterol hydro chloride (CL), which are tw o synthetic β-agonists, are. A rapid and simple high-performance liquid chromatography-UV method was developed for the separation and quantification of salbutamol, ractopamine, and clenbuterol in pork. A mixture of acetonitrile-formic acid-ammonium acetate was used as the mobile phase to separate three β-agonists on a C18 column with gradient. The detection limit of clenbuterol in pork by this method is 42 ng/g, which can realize the crude screening of pork containing clenbuterol in the market. Compared to previous studies [41], both the detection limit and reproducibility were improved. However, compared with traditional liquid chromatography, the detection limit is still higher. Clenbuterol (Clen) has been around for over 50 years. In the medical field, Clen is predominantly used as a nasal decongestant and bronchodilator for patients with respiratory health conditions like asthma. However, it is in bodybuilding that Clen really makes its mark as a anabolic-androgenic steroid (AAS). Clenbuterol (CB) is a synthetic β -receptor agonist which can be used to improve carcass leanness in swine, but its residues in pork also pose health risks. In this report, surface-enhanced Raman scattering (SERS) technology was used to achieve rapid detection and identification of clenbuterol hydrochloride (CB) residues
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